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Detailed analysis of atpenin A5 and phenformin cytotoxicity and inhibition of metabolic activity in various cell lines As predicted from our modeling approach as tier 1 targets, atpenin A5 at 20 µM and phenformin at 100 µM do not show toxicity in endpoint measurements based on total cell counting when Calu-3, CaCo-2, A549, and Huh7.5 cells were exposed to the compounds for 72 h (Fig

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These results suggested that RGSH could ameliorate AKI by inhibiting ferroptosis, which indicates RGSH as a promising therapeutic strategy for the treatment of AKI